Titlebook: Electroporation and Sonoporation in Developmental Biology; Harukazu Nakamura Book 2009 Springer-Verlag Tokyo 2009 Expression.RNA.Tempo.Ver

認(rèn)識(shí)

Single Cell Electroporation Method for Mammalian CNS Neurons in Organotypic Slice Culturesruber et al., 2001; O‘Brien et al., 2001; Ma et al., 2002; Sahly et al., 2003). In particular, the electroporation technique was proved as a valuable tool, since it can be applied to a wide range of tissues and cell types with little toxicity and can be performed with relative technical easiness. Mo

deciduous

加花粗鄙人

Gene Transfer into Mouse Haemogenic Sites, as a Mean to Functionally Approach the Control of Mesoderaintenance of haematopoiesis (Cumano and Godin, 2007)..In lower vertebrate species, such as Avian and Amphibian, the independent generation of extra- and intra-embryonic precursors pools, as well as the transient nature of the extra-embryonic one, was demonstrated through the use of interspecific ch

機(jī)械

Electroporation of Embryonic Kidney Explantsring RNA (Davies et al., 2004), by treating with pharmaceutical agents (Tang et al., 2002), or by using viral transduction to overexpress mutant proteins (Favre et al., 2000). More recently, microinjection followed by electroporation has been used to successfully express plasmid DNA encoding protein

obviate

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Debility

International Cases of Corporate Governancehickens has provided numerous novel insights into the understanding of early development in vertebrates, making the chicken a unique model animal..One of few shortfalls of the original technique has been that expression of electroporated genes does not persist for a long period of time probably beca

思考而得

tariff

International Cell Biology 1980–1981ion-mediated transgenesis. However, as long as the PSM was targeted, transgenesis was not successful for unknown reasons. Several years ago, we achieved somitic transgenesis by targeting PSM precursors (presumptive somitic cells) of earlier embryos, the cells residing in the epiblast of the anterior

Arctic

International Citizens‘ Tribunalsoses, a whole embryo culture system, originally invented by Dennis A. T. New (New, 1955), and its derivatives (Flamme, 1987; Sundin and Eichele, 1992; Stern, 1993; Chapman et al., 2001) have been widely used..Here we describe a method of electroporation for the early chick embryos using the . whole-