Titlebook: Electroporation and Sonoporation in Developmental Biology; Harukazu Nakamura Book 2009 Springer-Verlag Tokyo 2009 Expression.RNA.Tempo.Ver

善于騙人

Temporal Control of Gene Expression by Combining Electroporation and the Tetracycline Inducible Systplication of a small current through the targeted area. To date, all three germ layers — endoderm, mesoderm and ectoderm — as well as an increasing number of differentiated structures have been efficiently transfected (Dubrulle et al., 2001; Grapin-Botton et al., 2001; Itasaki et al., 1999; Luo and

制造

In Ovo Electroporation for Targeting the Somitic Mesodermion-mediated transgenesis. However, as long as the PSM was targeted, transgenesis was not successful for unknown reasons. Several years ago, we achieved somitic transgenesis by targeting PSM precursors (presumptive somitic cells) of earlier embryos, the cells residing in the epiblast of the anterior

alcoholism

Method of Electroporation for the Early Chick Embryooses, a whole embryo culture system, originally invented by Dennis A. T. New (New, 1955), and its derivatives (Flamme, 1987; Sundin and Eichele, 1992; Stern, 1993; Chapman et al., 2001) have been widely used..Here we describe a method of electroporation for the early chick embryos using the . whole-

BILK

Mhc-Molecule

Electroporation into the Limb: Beyond Misexpressionn formation during limb bud stages. Following establishment of these axes, one can visually recognize condensation of cartilages. Muscles, tendons, and neurons migrate and differentiate after cartilage formation. Because the stages and events are easily recognized morphologically and in detail, it i

Compass

Retroviral Vector-Mediated Gene Transfer into the Chick Optic Vesicle by In Ovo Electroporationonal analysis of genes has become possible in combination with classical techniques of developmental biology and neurobiology..One general method is replication-competent retroviral particle-mediated gene transfer in the chick embryo (Morgan and Fekete, 1996). After the infection of viral particles,

orient

Clonal and Widespread Gene Transfer by Proviral Electroporation for Analysis of Brain Laminar Format1; Sugiyama and Nakamura, 2003; Huber et al., 2008)..In spatial terms, widespread gene transfer by electroporation has provided an efficient way to unveil a new gene function on a given tissue (Nakamura and Funahashi, 2001). On the other hand, the spatial precision of gene transfer also has been add

媒介

Morose

In Utero Electroporation: Assay System for Migration of Cerebral Cortical Neuronsal., 2002). In utero electroporation of other sites in the brain, including the hippocampus (Navarro-Quiroga et al., 2007), cerebral basal ganglia (Borrell et al., 2005; Nakahira et al., 2006), cortical hem (Takiguchi-Hayashi et al., 2004), and dorsal thalamus (Bonnin et al., 2007), has recently bee

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