Titlebook: Drugs of Abuse; Neurological Reviews John Q. Wang Book 2003 Humana Press 2003

清楚說話

Heresy

Dorsal-Kyphosis

anaphylaxis

MARS

Analysis of mRNA Expression Using Double , Hybridization Labeling with Isotopic and Nonisotopic Prob example, acute or chronic exposures to psychostimulants, cocaine and amphetamine, increased basal levels of mRNA and protein products of immediate early genes (c-., ., Δ., etc.) in the rodent striatum (.–.). Similarly, acute administration of cocaine and amphetamines induced robust mRNA expression

思想靈活

Quantification of mRNA in Neuronal Tissue by Northern Analysiss spliced; that is, predetermined sequences (introns) are cut out of the transcript. If certain introns serve as either exons or introns, “alternative splicing” can occur and one gene can give rise to several different size RNA transcripts. The spliced RNA, the mRNA, is released into the cytosol and

integral

Analysis of Gene Expression in Striatal Tissue by Multiprobe RNase Protection Assay-term neurological effects of drugs of abuse. Before beginning such an investigation, however, one must first determine the method of RNA analysis most applicable to the problem at hand. The four most commonly used techniques include . hybridization, Northern blot, reverse transcriptase-polymerase c

alliance

abysmal

率直

Application of TaqMan RT-PCR for Real-Time Semiquantitative Analysis of Gene Expression in the Striaon in tissues of the central nervous system (CNS) including the striatum. For example, TaqMan has been used to profile mRNA distribution patterns across the brain for γ-aminobutyric acid-. (GABA.) receptor subunits (.), 5-hydroxytryptamine. (5-HT.) receptor splice variants (.), novel G-protein-coupl