Titlebook: Drosophila; Methods and Protocol Christian Dahmann Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive

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,Bet?ubungsmittelgesetz. (Opiumgesetz),y of these breakthroughs have been based on the identification of novel gene functions in large-scale genetic screens. However, conventional forward-genetic screens have been limited by the random nature of mutagenesis and difficulties in mapping causal mutations, while reverse-genetic RNAi screens

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Die Verdauungsvorg?nge bei Protozoene transcription by introducing genes on exogenous vectors or by using the CRISPR/dCas9-based transcriptional activation system. To date, the most efficient CRISPR/dCas9-based transcriptional activator is the Synergistic Activation Mediator (SAM) system whereby three different transcriptional activat

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https://doi.org/10.1007/978-3-642-91037-1xtraordinary insight into the mechanisms underlying normal and pathological development. The analysis of molecular determinants involved in the acquisition of cell identity requires gaining experimental access to precise parts of cell lineages. Recently, we have developed CaSSA and CLADES, a new tec

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Die Nahrungsaufnahme bei Protozoenand homeostasis in .. In contrast to other techniques, that indirectly interfere with proteins via their nucleic acids (CRISPR, RNAi, etc.), protein binders permit direct and acute protein manipulation. Since the first use of a nanobody in . a decade ago, many different applications exploiting prote

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B. P. Babkin,G. Bergmann,R. Winklerdifferent from its target. This method induces the binding of the target protein to the anchor by either the addition of rapamycin to . food or cell media. Rapamycin mediates the formation of a ternary complex between the anchor, which is tagged with the FK506-binding protein (FKBP12), and the targe

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