Titlebook: Drosophila; Methods and Protocol Christian Dahmann Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive

flavonoids

Analysis of Single-Cell Transcriptome Data in ,q data. In this book chapter we want to give guidance on analyzing droplet-based scRNA-seq data from .. We will initially describe the preprocessing commonly done for ., point out possible downstream analyses, and finally highlight computational methods developed using . scRNA-seq data.

obviate

CRISPR-Based Transcriptional Activation in ,ion domains are directly fused to dCas9 and MS2 phage Coat Protein (MCP), respectively, and the system in . is named flySAM. Here we describe the effective and convenient transcriptional activation system, flySAM, starting from vector construction, microinjection, and transgenic fly selection to the phenotypic analysis.

Charade

Tracing and Manipulating , Cell Lineages Based on CRISPR: CaSSA and CLADES,hnology based on CRISPR that allows targeting and labeling specific lineage branches. Here we discuss how to better exploit this technology for lineage studies in ., with an emphasis on neuronal specification.

Barter

駕駛

Optochemical Control of Cell Contractility in , Embryos,erence with single-cell resolution and sub-minute timescale temporal control. Here we characterize an optochemical approach of Ca. uncaging to control cell contractility in . embryos. We describe in detail the method of sample preparation, microinjection, Ca. uncaging, and data analysis.

切碎

圣歌

調(diào)情

Die Nahrungsaufnahme bei Protozoenn protein binders in .. We describe their key features and provide an overview of the available reagents. Finally, we briefly explore the future avenues that protein binders might open up and thus further contribute to better understand development and homeostasis of multicellular organisms.

cathartic

SUGAR