Titlebook: Directed Enzyme Evolution: Advances and Applications; Miguel Alcalde Book 2017 Springer International Publishing AG 2017 biocatalysis.biom

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etidronate

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Recent Advances in Directed Phytase Evolution and Rational Phytase Engineering,gains, oilseeds, and legumes and is indigestible by monogastric animals such as poultry and swine. Supplementation of phytase in animal feed proved to improve animal nutrition and decrease phosphorous pollution. Several phytases were discovered in the last century, and today a highly competitive mar

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Strain Development by Whole-Cell Directed Evolution,traits with hundreds of genetic determinants and for organisms with few genetic tools. Directed evolution mimics natural evolution in the laboratory via iterative cycles of diversity generation and functional selection or screening to isolate evolved mutants with desirable phenotypes. In this chapte

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Resurrected Ancestral Proteins as Scaffolds for Protein Engineering,es in protein scaffolds for laboratory-directed evolution and molecular design. Furthermore, the two features are not necessarily incompatible, and proteins may simultaneously be promiscuous/flexible and highly stable. In fact, it appears plausible that the combination of the two features was not un

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Molecular Modeling in Enzyme Design, Toward In Silico Guided Directed Evolution,lysis. Although this technique does not require any structural/mechanistic knowledge of the system, the frequency of improved mutations is usually low. For this reason, computational tools are increasingly used to focus the search in sequence space, enhancing the efficiency of laboratory evolution.