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Titlebook: Directed Enzyme Evolution: Advances and Applications; Miguel Alcalde Book 2017 Springer International Publishing AG 2017 biocatalysis.biom

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發(fā)表于 2025-3-21 19:59:03 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Directed Enzyme Evolution: Advances and Applications
編輯Miguel Alcalde
視頻videohttp://file.papertrans.cn/281/280661/280661.mp4
概述Offers a comprehensive view on directed evolution in the biotechnological ground.Presents a state of the art summary of the methodical advances in this thrilling research field.Highlights the most sig
圖書封面Titlebook: Directed Enzyme Evolution: Advances and Applications;  Miguel Alcalde Book 2017 Springer International Publishing AG 2017 biocatalysis.biom
描述.This book focuses on some of the most significant advances in enzyme engineering that have been achieved through directed evolution and hybrid approaches. On the 25th anniversary of the discovery of directed evolution, this volume is a tribute to the pioneers of this thrilling research field, and at the same time provides a comprehensive overview of current research and the state of the art..Directed molecular evolution has become the most reliable and robust method to tailor enzymes, metabolic pathways or even whole microorganisms with improved traits. By mirroring the Darwinian algorithm of natural selection on a laboratory scale, new biomolecules of invaluable biotechnological interest can now be engineered in a manner that surpasses the boundaries of nature..The volume is divided into two sections, the first of which provides an update on recent successful cases of enzyme ensembles from different areas of the biotechnological spectrum, including tryptophan synthases, unspecific peroxygenases, phytases, therapeutic enzymes, stereoselective enzymes and CO2-fixing enzymes. This section also provides information on the directed evolution of whole cells. The second section of the b
出版日期Book 2017
關(guān)鍵詞biocatalysis; biomolecular screening; industrial biotechnology; protein engineering; semi-rational appro
版次1
doihttps://doi.org/10.1007/978-3-319-50413-1
isbn_softcover978-3-319-84395-7
isbn_ebook978-3-319-50413-1
copyrightSpringer International Publishing AG 2017
The information of publication is updating

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沙發(fā)
發(fā)表于 2025-3-22 00:02:42 | 只看該作者
Discussion and Framework Development,y mutant libraries requiring minimal screening effort. This chapter constitutes a critical analysis of recent developments. Saturation mutagenesis at sites lining the binding pocket using highly reduced amino acid alphabets has emerged as the superior approach for evolving stereoselectivity.
板凳
發(fā)表于 2025-3-22 03:17:58 | 只看該作者
Book 2017ches. On the 25th anniversary of the discovery of directed evolution, this volume is a tribute to the pioneers of this thrilling research field, and at the same time provides a comprehensive overview of current research and the state of the art..Directed molecular evolution has become the most relia
地板
發(fā)表于 2025-3-22 07:14:53 | 只看該作者
Recent Advances in Directed Evolution of Stereoselective Enzymes,y mutant libraries requiring minimal screening effort. This chapter constitutes a critical analysis of recent developments. Saturation mutagenesis at sites lining the binding pocket using highly reduced amino acid alphabets has emerged as the superior approach for evolving stereoselectivity.
5#
發(fā)表于 2025-3-22 11:21:27 | 只看該作者
Konstruktionsvalidierung durch Simulation, attributes of this versatile enzyme while reflecting on the directed evolution campaigns recently followed in our laboratory that set out to enhance the functional expression of UPO in yeast and improve the activity, as well as approximating its properties to the required industrial standards.
6#
發(fā)表于 2025-3-22 16:36:38 | 只看該作者
7#
發(fā)表于 2025-3-22 20:52:36 | 只看該作者
https://doi.org/10.1007/978-3-658-03047-6iversity only. We describe and critically review recent advances in the methods for DNA assembly, random mutagenesis, focused mutagenesis, and DNA recombination. We also identify trends in these areas and highlight commercial kits that are developed to streamline and expedite these molecular biology techniques.
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發(fā)表于 2025-3-22 23:18:31 | 只看該作者
https://doi.org/10.1007/978-3-531-94062-5modeling, discussing their strengths, limitations, and directions. In addition, we suggest a possible future template for . directed evolution where we underline two main points: a hierarchical computational protocol combining several different techniques and a synergic effort between simulations and experimental validation.
9#
發(fā)表于 2025-3-23 05:05:38 | 只看該作者
Molecular Modeling in Enzyme Design, Toward In Silico Guided Directed Evolution,modeling, discussing their strengths, limitations, and directions. In addition, we suggest a possible future template for . directed evolution where we underline two main points: a hierarchical computational protocol combining several different techniques and a synergic effort between simulations and experimental validation.
10#
發(fā)表于 2025-3-23 07:53:21 | 只看該作者
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