Titlebook: Developmental Biology Protocols; Volume III Rocky S. Tuan,Cecilia W. Lo Book 2000 Humana Press 2000

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mRNA and Protein Co-Localization on Tissue Sections by Sequential, Colorimetric , Hybridization and ipts using a labeled nucleotide probe and 2. immunohistochemistry (IMH), which detects protein gene products by means of labeled primary or secondary antibodies. Because these methods may be, and often are, done with whole-mount specimens to obtain resolution at the cellular level, they are performe

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Multicolor Whole-Mount , Hybridization. Since its introduction into developmental biology (.), this procedure has become an indispensable tool to investigate gene expression; initial protocols employed tritiated probes. These procedures required very long exposure times and allowed the detection of transcripts only on tissue sections. T

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Guide to Teaching Puzzle-based Learning expression to be clearly discerned (.). Similar to conventional in situ hybridization, one can determine the precise cellular distribution of expression by embedding and sectioning embryos following WMISH. For these reasons WMISH has become an essential and standard tool for studying gene expressio

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Bo Zhou,Curtis Maines,Stephen Tang,Qi Shi, however, where the placement of the large GFP probe has interfered with the nearby domains of proteins. For example, coupling GFP to the N-terminal region of alpha-actinin, near the actin-binding domain resulted in a GFP probe that could not bind actin, whereas GFP linked to the C-terminus of alph

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Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins, however, where the placement of the large GFP probe has interfered with the nearby domains of proteins. For example, coupling GFP to the N-terminal region of alpha-actinin, near the actin-binding domain resulted in a GFP probe that could not bind actin, whereas GFP linked to the C-terminus of alph