Titlebook: Developmental Biology Protocols; Volume III Rocky S. Tuan,Cecilia W. Lo Book 2000 Humana Press 2000

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書目名稱Developmental Biology Protocols影響因子(影響力)




書目名稱Developmental Biology Protocols影響因子(影響力)學(xué)科排名




書目名稱Developmental Biology Protocols網(wǎng)絡(luò)公開度




書目名稱Developmental Biology Protocols網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Developmental Biology Protocols被引頻次




書目名稱Developmental Biology Protocols被引頻次學(xué)科排名




書目名稱Developmental Biology Protocols年度引用




書目名稱Developmental Biology Protocols年度引用學(xué)科排名




書目名稱Developmental Biology Protocols讀者反饋




書目名稱Developmental Biology Protocols讀者反饋學(xué)科排名

范例

Clonal Analysis in the Examination of Gene Function in ,lineage, fate determination, autonomy of gene action and pattern formation (., .). Clonal analysis has been particularly useful for the study of genes that would be lethal in a homo-zygous mutant state; this approach also makes it possible to recover essential genes in mosaic screens (.).

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Application of Antisense Oligodeoxynucleotides in Developing Chick EmbryosOne technique used for perturbing the level of expression of a specific gene is the application of gene-specific antisense oligodeoxyribonucleotides (ODNs). ODNs are a quick, affordable, and effective means to “knock down” the expression of a gene in order to learn more of its function in vivo. It i

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Gene Expression Analyzed by Ribonuclease Protection Assays as well as for the analysis of mRNA and gene structure (.). This solution hybridization approach is at least 10-fold more sensitive than Northern blot analysis, and thus is useful for the evaluation of low-abundance mRNAs. Furthermore, the greater stability of the RNA duplex structure over RNA-DNA

observatory

Relative Reverse Transcription-Polymerase Chain ReactionPCR), Northern blot analysis, primer extension, and RNA protection assays (.). Because of the PCR amplification step, RT-PCR has the important advantage that little RNA is required. In addition, RT-PCR is a relatively simple procedure that does not have an absolute requirement for the use of radiois

observatory

Gene Expression Analysis Using Quantitative Reverse Transcription-Polymerase Chain Reaction and a Musuming task that necessitates the use of carefully controlled amplification procedures. Reverse transcription (RT) of the mRNA to cDNA followed by amplification using the polymerization chain reaction (RT-PCR) is widely used for simple detection purposes, but the procedure can be employed for accura

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Radio-Isotopic , Hybridization on Tissue Sectionsental studies. Different strategies are to be used for different purposes. Whole mount . hybridization allows the rapid global analysis of changes in the spatiotemporal patterns of gene expression. Its use is limited to early developmental stages owing to penetration problems with increasing size of