Titlebook: Deadenylation; Methods and Protocol Eugene Valkov,Aaron C. Goldstrohm Book 2024 This is a U.S. government work and not under copyright prot

synchronous

CLOWN

個阿姨勾引你

Eugene Valkov,Aaron C. GoldstrohmIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

VEN

Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/263953.jpg

Comedienne

Reconstitution of Human CCR4-NOT Complex from Purified Proteins and an Assay of Its Deadenylation Ainfected insect cells, purified using column chromatography, and reconstituted into a stable complex containing all eight core subunits. In addition, we describe the biochemical assay of deadenylation using the reconstituted complex.

物種起源

Transcriptome-Wide Analysis of mRNA Adenylation Status in Yeast Using Nanopore Sequencing,ics. However, direct RNA sequencing using the Oxford Nanopore Technologies (ONT) platform makes it possible to conduct transcriptome-wide analyses at the single-molecule level without the PCR bias introduced by other methods. In this chapter, we provide a protocol to measure both RNA levels and poly(A)-tail lengths in the yeast . using ONT.

演繹

Reconstitution of Human CCR4-NOT Complex from Purified Proteins and an Assay of Its Deadenylation Ainfected insect cells, purified using column chromatography, and reconstituted into a stable complex containing all eight core subunits. In addition, we describe the biochemical assay of deadenylation using the reconstituted complex.

百靈鳥

Yourself

–FER