Titlebook: Deadenylation; Methods and Protocol Eugene Valkov,Aaron C. Goldstrohm Book 2024 This is a U.S. government work and not under copyright prot

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書目名稱Deadenylation影響因子(影響力)




書目名稱Deadenylation影響因子(影響力)學科排名




書目名稱Deadenylation網(wǎng)絡(luò)公開度




書目名稱Deadenylation網(wǎng)絡(luò)公開度學科排名




書目名稱Deadenylation被引頻次




書目名稱Deadenylation被引頻次學科排名




書目名稱Deadenylation年度引用




書目名稱Deadenylation年度引用學科排名




書目名稱Deadenylation讀者反饋




書目名稱Deadenylation讀者反饋學科排名

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Embarking on the Digital Odysseyhe tail, and quantify transcript abundance analogous to traditional RNAseq methods. Here, we discuss the method for generating, sequencing, and primary analysis of poly(A) tail data from total RNA using the Pacific Biosciences Sequel platform.

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Quantification of Poly(A) Tail Length and Terminal Modifications Using Direct RNA Sequencing, cover how to prepare the libraries and perform the bioinformatics analysis to simultaneously determine the length of the transcripts’ poly(A) tails and detect the presence of terminal guanylation and uridylation.

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軌道

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https://doi.org/10.1007/978-3-031-70807-7inclusive full-length RNA isoform-sequencing (PAIso-seq), a method that can measure transcriptome-wide poly(A) tails from as low as nanogram level of total RNA based on the PacBio HiFi sequencing platform. The accurate length and base composition of poly(A) tails can be obtained along with the full-length cDNA.

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