Titlebook: DNA Replication; Methods and Protocol Sonya Vengrova,Jacob Dalgaard Book 2015Latest edition Springer Science+Business Media New York 2015 D

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Chinese Environmental Governancehich we regulate the activity of the barrier, how to synchronize cells for analysis of replication intermediates by 2D gel electrophoresis, and the use of a replication slippage assay to measure fork fidelity.

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Analyzing the Dynamics of DNA Replication in Mammalian Cells Using DNA Combing, This allows quantitative measurements of several salient features of chromosome replication dynamics, such as fork velocity, fork asymmetry, inter-origin distances, and global instant fork density. In this chapter we describe how to obtain this information from asynchronous cultures of mammalian cells.

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Chromatin Immunoprecipitation to Detect DNA Replication and Repair Factors,ated at chromosomal regions that are difficult to replicate. In this chapter, we describe a chromatin immunoprecipitation method to locate proteins required for DNA repair during DNA replication in the fission yeast .. This method can also easily be adapted to study replisome components or chromatin-associated factors.