Titlebook: DNA Replication; Methods and Protocol Sonya Vengrova,Jacob Dalgaard Book 2015Latest edition Springer Science+Business Media New York 2015 D

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Chinese Entrepreneurs in Societynsequences. Here, we describe an assay to quantify stable ribonucleotide incorporation by DNA polymerases in vitro, and an assay to probe for ribonucleotides in each of the two DNA strands of the yeast nuclear genome.

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https://doi.org/10.1057/9781137492913, the study of DNA replication with multicolour flow analysis has lagged behind its use in mammalian cells. We present basic and advanced protocols for analysis of DNA replication in fission yeast by flow cytometry including whole cell, nuclear “ghosts,” two-color imaging with BrdU, and estimates of DNA synthesis using EdU.

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