Titlebook: DNA Modifications; Methods and Protocol Alexey Ruzov,Martin Gering Book 2021 The Editor(s) (if applicable) and The Author(s), under exclusi

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Detection of Low-Abundance DNA Modifications Using Signal Amplification-Based ImmunocytochemistryC, 5-hydroxymethyluracil, 5hmU) in mammalian DNA. This method can be employed for evaluation of the levels and nuclear distribution of DNA modifications and permits their colocalization with protein markers in animal cells.

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https://doi.org/10.1007/978-1-0716-0876-0Non-canonical DNA nucleotides; DNA demethylation; Mass Spectrometry; Antibody-based techniques; Bioinfor

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Nusa Zidaric,Mark Aagaard,Guang Gongn Translocation (TET) dioxygenases oxidize 5mC to 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine in metazoans and fungi. Moreover, two recent reports imply the existence of other species of modified cytosine in unicellular alga . and malaria parasite .. Here we provide an overview

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Lecture Notes in Computer Scienceng is the availability of techniques that permit sensitive and specific genome-wide mapping of DNA modifications in mammalian DNA. The last decade has seen the development of a vast arsenal of novel profiling approaches enabling epigeneticists to tackle research questions that were previously out of

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?etin Kaya Ko?,Sihem Mesnager,Erkay Sava?te. The continuing discovery of new modifications in phages suggest many more are waiting to be found. Thus, methods for the observation and characterization of noncanonical nucleosides are timely. We present here protocols for extraction of genomic DNA from bacteriophage particles, enzymatic hydrol

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