Titlebook: DNA Methylation Protocols; J?rg Tost Book 2018Latest edition Springer Science+Business Media, LLC 2018 DNA.cytosines.gene expression.chrom

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Tagmentation-Based Library Preparation for Low DNA Input Whole Genome Bisulfite Sequencingod covering the complete methylome. Alternative methods requiring less DNA than WGBS analyze only a minor portion of the methylome and do not cover important regulatory features like enhancers and noncoding RNAs. In tagmentation-based WGBS (TWGBS), several DNA and time-consuming steps of the convent

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Multiplexed Reduced Representation Bisulfite Sequencing with Magnetic Bead Fragment Size Selection sequenced. It allows researchers to target gene regions with particular CpG densities, thereby selecting the desired genomic contexts. Here, we describe an approach that uses magnetic beads to accomplish this selection. In addition, the use of indexed, methylated adapters enables up to 12 samples t

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Comprehensive Whole DNA Methylome Analysis by Integrating MeDIP-seq and MRE-seqg-based DNA methylation profiling provides an unprecedented opportunity to map and compare complete DNA CpG methylomes. These include whole genome bisulfite sequencing (WGBS), Reduced-Representation Bisulfite-Sequencing (RRBS), and enrichment-based methods such as MeDIP-seq, MBD-seq, and MRE-seq. An

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Digital Restriction Enzyme Analysis of Methylation (DREAM)n sequencing (NGS) technology. The method is based on sequential cuts of genomic DNA with a pair of restriction enzymes (.I and .I) at CCCGGG target sites. Unmethylated sites are first digested with .I. This enzyme cuts the sites in the middle at CCC^GGG, leaving behind blunt ended fragments. CpG me