Titlebook: Cytokine Protocols; Marc Ley Book 2004 Humana Press 2004

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Forschungsdesign und Verlauf der Studie,lls after antigenic stimulation by the influence of cytokines [interleukin 12 (IL-12)/interferon-γ (IFN-γ) in Th1 and IL-4/IL-13 in Th2] or costimulatory molecules (CD80/CD86) provided from antigen presenting cells (APC), and have several important immunological functions (.). Recently, several repo

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Detection of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level,ning techniques have been developed in our laboratory. The difficulties in these procedures lie in the critical steps of fixation and permeabilization of the cells in combination with a very careful selection of suitable cytokine- or chemokine-specific antibodies.

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Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/242632.jpg

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Large-Scale Generation of Plasmids that Express Type I Interferon, DNA vaccinations with plasmid cytokine adjuvants (.–.), tumor research (.), and treatment of infectious diseases (.,.). Small-scale plasmid preparations (approx 10–100 μg DNA) usually result in adequate yields, whereas large-scale (>1 mg DNA) preparations of plasmid DNA can result in disproportiona

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Identification of ,-Acting Factors by Electrophoretic Mobility Shift Assay,l transduction pathways, culminating in observable cellular functions, such as modulation of cell motility, alteration of cell proliferation rate, or induction of apoptosis. Understanding the molecular mechanisms that mediate these cellular functions is of critical importance as aberrations of these