| 書目名稱 | Cytokine Protocols | | 編輯 | Marc Ley | | 視頻video | http://file.papertrans.cn/243/242632/242632.mp4 | | 概述 | Includes supplementary material: | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | Because cytokines regulate many different bodily functions in living organisms, mainly at the level of cell-cell communications, there is great interest in understanding their mode of action. In Cytokine Protocols, established researchers, physicians, and clinicians present their best biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. Described in step-by-step detail to ensure successful experimental results, these protocols cover the large-scale generation and purification of plasmid DNA, the identification of DNA-protein interactions via the gel mobility shift assay, RNA-level phenomena, and the isolation and characterization of cytokines, cytokine-related proteins, and their interactions. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, i | | 出版日期 | Book 2004 | | 版次 | 1 | | doi | https://doi.org/10.1385/1592596673 | | isbn_softcover | 978-1-61737-269-8 | | isbn_ebook | 978-1-59259-667-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2004 |
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