Titlebook: Chromatin Immunoprecipitation; Methods and Protocol Franziska Greulich Book 2024 The Editor(s) (if applicable) and The Author(s), under exc

敏捷

Expertise

CUT&Tag for Efficient Epigenomic Profiling of Frozen Tissues,col details the steps to generate CUT&Tag libraries from fresh or frozen tissues. This CUT&Tag workflow has nine main steps: isolation of nuclei from tissues, binding of nuclei to Concanavalin A–coated beads, binding of the primary antibody, binding of the secondary antibody, binding pA-Tn5 adapter

要塞

Fierce

Single-Cell Histone Modification Profiling with Cell Enrichment Using sortChIC,tiation, the distribution of histone modifications is remodeled, resulting in cell type–specific patterns. In the past, their study was limited to abundant cell types that could be purified in necessary numbers. However, studying these cell type–specific dynamic changes in heterogeneous in vivo sett

DUST

燒烤

ChEC-Seq: A Comprehensive Guide for Scalable and Cost-Efficient Genome-Wide Profiling in ,nding locations in vivo, does not require antibodies or fixation, and provides genome-wide coverage at near nucleotide resolution..The core of this method is an MNase fusion of the target protein, which allows it, when triggered by calcium exposure, to cut DNA at its binding sites and to generate sm

一窩小鳥

Chromatin Immunoprecipitation in Adipose Tissue and Adipocytes: How to Proceed and Optimize the ProIP from adipocytes or frozen adipose tissue collection, isolation of nuclei, cross-linking of protein-DNA complexes, chromatin shearing, immunoprecipitation, and DNA purification. We also discuss critical steps for optimizing the experiment to perform a successful ChIP in lipid-rich cells/tissues.

Mirage

Bioinformatics Core Workflow for ChIP-Seq Data Analysis,t covers data processing and initial analytical steps of ChIP-seq data. We provide a step-by-step protocol of the commands as well as a fully assembled Snakemake workflow. Along the protocol, we discuss key tool parameters, quality control, output reports, and preliminary results.

良心

Surgeon