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Titlebook: Chromatin Immunoprecipitation; Methods and Protocol Franziska Greulich Book 2024 The Editor(s) (if applicable) and The Author(s), under exc

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發(fā)表于 2025-3-21 19:08:56 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Chromatin Immunoprecipitation
副標題Methods and Protocol
編輯Franziska Greulich
視頻videohttp://file.papertrans.cn/243/242117/242117.mp4
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Chromatin Immunoprecipitation; Methods and Protocol Franziska Greulich Book 2024 The Editor(s) (if applicable) and The Author(s), under exc
描述.This comprehensive guide delves into the protocols and strategies for investigating gene regulation both experimentally and computationally. It focuses on the use of Chromatin Immunoprecipitation (ChIP) coupled with next-generation sequencing, providing a robust framework for profiling DNA-binding proteins, examining DNA footprinting, and analyzing histone modifications. As part of the highly successful .Methods in Molecular Biology. series, this book offers introductions on the topics covered, detailed lists of the necessary materials and reagents, step-by-step laboratory protocols that are easy to follow, and expert tips for troubleshooting and avoiding known pitfalls...?..Authoritative and practical, .Chromatin Immunoprecipitation: Methods and Protocols. is the perfect resource for the new wave of genome biologists looking to master ChIP techniques..
出版日期Book 2024
關鍵詞Next-generation sequencing; ChIP-seq; DNA sequences; Transcription factors; Bioinformatics; CUT&RUN
版次1
doihttps://doi.org/10.1007/978-1-0716-4071-5
isbn_softcover978-1-0716-4073-9
isbn_ebook978-1-0716-4071-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Chromatin Immunoprecipitation in Adipose Tissue and Adipocytes: How to Proceed and Optimize the Prontrol of specific transcriptional factors or co-regulators at loci-specific or pan-genomic levels..Here we provide a reliable method for processing ChIP from adipocytes or frozen adipose tissue collection, isolation of nuclei, cross-linking of protein-DNA complexes, chromatin shearing, immunoprecipi
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Sequential ChIP-Seq, assay determines the in vivo co-localization of two proteins to the same genomic locus. In this chapter, we combine the two protocols in Sequential ChIP-Seq, a method for identifying genome-wide sites of in vivo protein co-occupancy.
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Estrogen Receptor Chromatin Profiling by CUT&RUN,f gene transcription. Steroid hormones activate nuclear hormone receptor (HR), transcription factors (TFs), which bind DNA in a tissue- and cell type–specific manner to influence cellular function. Identifying the genomic binding sites of HRs is essential to understanding mechanisms of hormone signa
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Cleavage Under Targets and Release Using Nuclease (CUT&RUN) in Macrophages, valuable for the characterization of the binding of transcription factors or co-regulators genome wide. Furthermore, it can be used for epigenetic profiling, chromatin accessibility assessment, and identification of regulatory elements. Compared to the more commonly used chromatin immunoprecipitati
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