Titlebook: Cryo-Electron Tomography; Structural Biology i Friedrich F?rster,Ariane Briegel Book 2024 The Editor(s) (if applicable) and The Author(s),

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Cryo-Focused Ion Beam Milling of Cells,ity to frozen cells. Preparing samples using FIB milling of frozen-hydrated cells at cryogenic temperatures is known as cryo-FIB milling, which has emerged as one of the gold-standard techniques for cellular cryo-ET to generate molecular landscapes inside cells. In this chapter, we present a brief h

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In Situ Cryo-Electron Tomography and Advanced Micromanipulator Techniques,nd higher throughout. The following chapter provides a topical overview of this new and rapidly developing technique. We introduce the basic principles of cryo-lift out and briefly summarize the technological and biological results so far.

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Beam-Induced Motion Mechanism and Correction for Improved Cryo-Electron Microscopy and Cryo-Electro” response, having both a repeated elastic component and a plastic deformation. The elastic deformation represents the portion that can be fully relaxed when the electron beam is turned off at the end of the exposure for each tilted image. By contrast, the plastic deformation decays exponentially wi

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Detection and Interpretation of Cellular Structures in Tomograms: Segmentation, Localization and Spachine learning and advanced algorithms, and discuss challenges posed by the recent developments of deep learning methods. New approaches for localization of molecular complexes and spatial analysis are expected to provide precise molecular maps of diverse cellular compartments, elucidate the organi

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Electron Tomography of Cryo-Fixed and Resin-Embedded Samples,g a wider context with equal detail. Electron tomography can also be performed on serial sections which allows entire cells or tissue volumes to be reconstructed at high resolution. Further, high precision correlative microscopy workflows can be implemented, permitting to combine information on mole

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Book 2024 from the fundamental principles of imaging to sample preparation, data analysis,and data sharing within the scientific community. It serves as a valuable resource for the next generation of structural biologists, making it suitable both for undergraduate students studying biochemistry, biophysics,

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1571-4853 sharing within the scientific community. It serves as a valuable resource for the next generation of structural biologists, making it suitable both for undergraduate students studying biochemistry, biophysics, 978-3-031-51173-8978-3-031-51171-4Series ISSN 1571-4853 Series E-ISSN 2542-9566

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