Titlebook: Corneal Regeneration; Methods and Protocol Mark Ahearne Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 limbal

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Corneal Regeneration978-1-0716-0599-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Isolation and Culture of Corneal Stromal Stem Cells,research, and potentially in the development of clinically relevant cellular therapies. Postmortem corneal tissue obtained from otherwise discarded material after keratoplasties is oftentimes the source of the cells for ex vivo research. Relatively easy to isolate and cultivate as well as inexpensiv

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Primary Culture of Cornea-Limbal Epithelial Cells In Vitro,e in vitro expanded cornea-limbal epithelial cell cultures are rich in stem cells for cornea, and hence can be used as a cell therapy for cornea-limbal deficiency. This chapter details the primary culture of these cornea-limbal cells, which can be used as model for further studies of the cornea surf

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Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant,ll deficiency. The protocols used to expand limbal epithelial cells in vitro need to be optimized, since the scarcity of human ocular tissue donors is limiting the potential use of this procedure. Here, we describe a method to consecutively expand a single human limbal explant. With this method it i

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A Guide to the Development of Human CorneaOrganoids from Induced Pluripotent Stem Cells in Culture,ated production of extracellular matrix, epithelial differentiation, and endothelial cell expansion to produce a highly transparent tissue. Here we describe the production of multilayered three-dimensional organoids from human-induced pluripotent stem cells. These organoids have the potential for mu

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Gene Editing for Corneal Stromal Regeneration, an RNA-guided nuclease in which the sequence of the RNA can be altered to match the desired target. However, care must be taken in target choice and RNA guide design to ensure both maximum on-target and minimum off-target activity. The cornea is an ideal tissue for gene therapy due to its small sur