Titlebook: Corneal Regeneration; Methods and Protocol Mark Ahearne Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 limbal

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Substitution

CLOWN

Mark AhearneIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/238270.jpg

braggadocio

Dynamic Texturing in Virtual Environmentsresearch, and potentially in the development of clinically relevant cellular therapies. Postmortem corneal tissue obtained from otherwise discarded material after keratoplasties is oftentimes the source of the cells for ex vivo research. Relatively easy to isolate and cultivate as well as inexpensiv

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Granular

Tornadoes and Other Whirling Winds,e in vitro expanded cornea-limbal epithelial cell cultures are rich in stem cells for cornea, and hence can be used as a cell therapy for cornea-limbal deficiency. This chapter details the primary culture of these cornea-limbal cells, which can be used as model for further studies of the cornea surf

arousal

Some More Advanced STEM Problems,ll deficiency. The protocols used to expand limbal epithelial cells in vitro need to be optimized, since the scarcity of human ocular tissue donors is limiting the potential use of this procedure. Here, we describe a method to consecutively expand a single human limbal explant. With this method it i

pessimism

Some More Advanced STEM Problems,ated production of extracellular matrix, epithelial differentiation, and endothelial cell expansion to produce a highly transparent tissue. Here we describe the production of multilayered three-dimensional organoids from human-induced pluripotent stem cells. These organoids have the potential for mu

collagen

Some More Advanced STEM Problems, an RNA-guided nuclease in which the sequence of the RNA can be altered to match the desired target. However, care must be taken in target choice and RNA guide design to ensure both maximum on-target and minimum off-target activity. The cornea is an ideal tissue for gene therapy due to its small sur