Titlebook: Clostridium difficile; Methods and Protocol Peter Mullany,Adam P. Roberts Book 2010 Springer Science+Business Media, LLC 2010 DNA.Microarra

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Comparative Genome Analysis of , Using DNA Microarrays Nevertheless, whole-scale genome sequencing remains an expensive endeavour and such comparisons are limited to only a handful of strains. DNA microarrays represent an alternative technology for whole genome comparisons enabling a “birds eye view” of all the genes absent or present in a given genome

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ClosTron-Targeted Mutagenesis chromosome via a single crossover event. As an alternative, recombination-independent strategies have been devised that are reliant upon a re-targeted group II intron. One element in particular, the ClosTron, provides the facility for the positive selection of insertional mutants. The generation of

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Clostridium difficile978-1-60327-365-7Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Peter Mullany,Adam P. RobertsServes as a comprehensive guide to most of the current techniques for manipulating and analyzing C. difficile.Written by scientists who have pioneered modern C. difficile research.Brings together in o

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E. Straube,G. Polzer,G. Buchkremerged from an obscure, and often under-researched, bacterium to one of major clinical importance, at least in industrialized nations. Coupled with the increased interest in this organism researchers now have a greater understanding of its genetic content and molecular epidemiology; a direct consequenc

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Ch. Beal Anderson,L. Ereshefskylitis or toxic megacolon. The disease arises as a major complication of antibiotic therapy and is most commonly acquired in hospital. The laboratory investigation of faecal samples is supportive of a clinical suspicion that a patient has the disease. Currently the mainstay of diagnosis is the demons

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