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Titlebook: Clostridium difficile; Methods and Protocol Peter Mullany,Adam P. Roberts Book 2010 Springer Science+Business Media, LLC 2010 DNA.Microarra

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書目名稱Clostridium difficile
副標題Methods and Protocol
編輯Peter Mullany,Adam P. Roberts
視頻videohttp://file.papertrans.cn/229/228386/228386.mp4
概述Serves as a comprehensive guide to most of the current techniques for manipulating and analyzing C. difficile.Written by scientists who have pioneered modern C. difficile research.Brings together in o
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Clostridium difficile; Methods and Protocol Peter Mullany,Adam P. Roberts Book 2010 Springer Science+Business Media, LLC 2010 DNA.Microarra
描述.Clostridium difficile., a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide. In .Clostridium difficile: Methods and Protocols., expert researchers bring together the most recently developed methods for studying the organism, including techniques involving isolation, molecular typing, genomics, genetic manipulation, and the use of animal models.? Written in the highly successful .Methods in Molecular Biology.? series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls...?..Authoritative and cutting-edge, .Clostridium difficile: Methods and Protocols. serves as an ideal guide for scientists now in a position to gain an in-depth understanding of how this organism is transmitted and how it causes disease..
出版日期Book 2010
關(guān)鍵詞DNA; Microarray; PCR; Toxin; antibiotic-associated disease; antibiotics; genes; molecular biology; infectiou
版次1
doihttps://doi.org/10.1007/978-1-60327-365-7
isbn_softcover978-1-4939-5701-9
isbn_ebook978-1-60327-365-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2010
The information of publication is updating

書目名稱Clostridium difficile影響因子(影響力)




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,Synopsis: Schizophrenie — was ist das?,ell as its own. TcdE has high homologies with bacteriophage holin proteins. TcdC negatively regulates toxin synthesis by interfering with the RNA polymerase formed with TcdR. Therefore, TcdR and TcdC constitute specific regulators of toxin gene transcription thereby tightly regulating toxin synthesi
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Daniel Hell,Magret Fischer-Gestefeld Nevertheless, whole-scale genome sequencing remains an expensive endeavour and such comparisons are limited to only a handful of strains. DNA microarrays represent an alternative technology for whole genome comparisons enabling a “birds eye view” of all the genes absent or present in a given genome
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Was verursacht Schizophrenien?, chromosome via a single crossover event. As an alternative, recombination-independent strategies have been devised that are reliant upon a re-targeted group II intron. One element in particular, the ClosTron, provides the facility for the positive selection of insertional mutants. The generation of
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Isolation and Culture Techniques. This combination of tests should include culture (with toxin testing of the isolate), demonstration of toxin direct from the faeces and the detection of . antigen. This chapter outlines the methods used to establish the laboratory diagnosis of CDI and also includes the investigation of environment
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Multilocus Sequence Typing for ,eveals a clonal population structure. Mutational evolution of . is characterized, with point mutation generating new alleles at a frequency eightfold higher than recombinational exchange. Phylogenetic analysis shows that human and animal isolates do not cluster in distinct lineages, and that no hype
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