Titlebook: Circular RNAs; Methods and Protocol Christoph Dieterich,Argyris Papantonis Book 2018 Springer Science+Business Media, LLC, part of Springer

Fracture

Metamorphosis

審問

Detecting Circular RNAs by RNA Fluorescence In Situ Hybridization,need to be kept low. Here, we describe a FISH protocol coupled to high-precision localizations using a single fluorescently labeled probe spanning the circRNA junction; this allows circRNA detection in mammalian cells with high signal-to-noise ratios.

DUCE

Single-Molecule Fluorescence In Situ Hybridization (FISH) of Circular RNA CDR1as,f a circular RNA, CDR1as, simultaneously with an unrelated messenger RNA. The protocol can be adapted to circRNAs that coexist with overlapping, noncircular mRNA isoforms produced from the same genetic locus.

thyroid-hormone

考博

HATCH

Identification of circRNAs for miRNA Targets by Argonaute2 RNA Immunoprecipitation and Luciferase SNAs. The AGO2 RIP assay evaluates the potential of the interaction between circRNAs and miRNAs. The luciferase screening assay investigates the targeting miRNAs and the detail binding sites for a specific circRNA.

Control-Group

Book 2018ratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Circular RNAs: Methods and Protocols .aims to useful and informative for further study into this vital field.?.