Titlebook: Circular RNAs; Methods and Protocol Christoph Dieterich,Argyris Papantonis Book 2018 Springer Science+Business Media, LLC, part of Springer

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https://doi.org/10.1007/978-3-642-54508-5y to confirm the existence and to evaluate the size of individual circRNAs. Northern blotting probes can be radioactive isotope (.P) labeled, which is not environment-friendly and sometimes inconvenient to use. Here, we describe a nonradioactive northern blot protocol with digoxigenin-labeled probe

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https://doi.org/10.1007/978-3-642-54508-5f them exhibit low expression levels but some are extremely abundant. Typically, circRNAs are studied by RT-PCR-based assays, but for certain types of analyses this technique is not suitable. Circular RNA with repetitive exons (circular concatemers) has been observed by us and others when transientl

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https://doi.org/10.1007/978-3-642-54508-5n coding potential, currently classified as long noncoding RNAs (lncRNA). A distinct subgroup of lncRNAs is circular RNAs (circRNAs), which can be easily identified by unique junction reads, resulting from their biogenesis. CircRNAs are largely cytosolic and thought to either code for micro-peptides

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State Regulation: Achieving Ascendancy,As (miRNAs) and inhibit the activity of miRNAs. Alternatively, miRNAs could also directly target circRNAs and regulate the expression of circRNAs. Here we describe the Argonaute2 (AGO2) RNA immunoprecipitation (RIP) and luciferase screening assays to identify the interaction between circRNAs and miR

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