Titlebook: Cilia; Methods and Protocol Vito Mennella Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Sc

陳舊

Primary Human Nasal Epithelial Cell Culture,y tract. The current chapter details protocols from sample collection to culturing HNEC under air-liquid interface (ALI) conditions to generate well-differentiated, functional airway cultures including displaying beating motile cilia.

賠償

https://doi.org/10.1007/978-1-0716-3507-0Tissue development; Homeostasis; Ciliopathies; Ciliary proteins; Human disease; Toxicant exposure

Arrhythmia

978-1-0716-3509-4The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

慢慢啃

Vito MennellaIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

Expressly

誓言

Cilia978-1-0716-3507-0Series ISSN 1064-3745 Series E-ISSN 1940-6029

Locale

即席

Die Gauck-Beh?rde: das unm?gliche Amttion and cellular content in three dimensions at nanoscale resolution. Here, we describe the procedure to process and image an air-liquid interface culture of human or mouse airway epithelial cells for visualization of the multiciliated epithelium by SBF-SEM in vertical or horizontal cross section.

IDEAS

Wie gefestigt ist unsere Demokratie?living cells. Here, we describe experimental strategies for endogenous PCR-tagging of ciliary genes in human RPE1 cells and how image acquisition and analysis of the expressed fluorescently tagged proteins can be utilized to study the dynamic ciliary processes of intraflagellar transport and vesicular trafficking.

古老