Titlebook: Cilia; Methods and Protocol Vito Mennella Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Sc

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OMIT

ESO

Osteons

Book 2024f different ciliated tissues and their wide applicability in cilia biology. Chapters in this book primarily focus on methods to study multiciliated cells, and discuss topics such as SARS-CoV-2 infections of human primary nasal multiciliated epithelial cells; expansion microscopy of ciliary proteins;

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忘川河

AORTA

Regierungssysteme in Mittel- und Osteuropaodified inserts – referred to as mucociliary transport devices (MCTDs) – are simple to prepare and result reproducibly in a high percentage of cultures demonstrating complete circular transport (CCT).

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Immunolabel-First-Expand-Later Expansion Microscopy Approach Using Stable STED Dyes,ing their use in an immunolabel-first-expand-later approach. Our protocol overcomes the frequent technical limitation of poor, unreproducible binding of primary antibodies to proteins after denaturation. We demonstrate the applicability of this approach by analyzing both a centriole appendage protein Cep164 and a ciliary protein ARL13B.

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Mucociliary Transport Device Construction and Application to Study Mucociliary Clearance,odified inserts – referred to as mucociliary transport devices (MCTDs) – are simple to prepare and result reproducibly in a high percentage of cultures demonstrating complete circular transport (CCT).