Titlebook: Chromaffin Cells; Methods and Protocol Ricardo Borges Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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https://doi.org/10.1057/9780230339378xidized two electrons (e.) that are recorded as an electrical current. Amperometry is an easy-to-use and noninvasive technique for cells (unlike patch-clamp techniques for measuring membrane capacitance) and has been widely used to monitor online catecholamine release from perifused bovine chromaffin cell populations.

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https://doi.org/10.1007/978-1-349-27445-1 granules, or stress in the endoplasmic reticulum. In this chapter, we describe current methods to study mitochondrial, endoplasmic reticulum, and secretory vesicle calcium homeostasis in living chromaffin cells using engineered targeted aequorins.

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Liberalism and the Challenge of Pluralismpacitance recordings. Precise stimulation of exocytosis is achieved by local pressure application or voltage-clamp depolarizations. While the chapter is focused on insulin-secreting cells as an accessible and disease-relevant model system, the methodology is applicable to a wide variety of secretory cells including chromaffin and PC12 cells.

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Introduction: Hoping for the Future,the steps necessary to process the adrenochromaffin tissue for the transmission electron microscopy; this protocol is suitable for any kind of adrenal tissue, and personally we used it in mammals, reptiles, and amphibians.

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The Future Society and the Transition to It in real time. Here we depict a method of using the state-of-the-art STED microscopy to image these membrane dynamics in bovine chromaffin cells. This method can potentially be applied to study other membrane structure dynamics in other cell model system.

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Fretful

Transmission Electron Microscopy: A Method for Studying the Adrenal Chromaffin Cellsthe steps necessary to process the adrenochromaffin tissue for the transmission electron microscopy; this protocol is suitable for any kind of adrenal tissue, and personally we used it in mammals, reptiles, and amphibians.

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