Titlebook: Chromaffin Cells; Methods and Protocol Ricardo Borges Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/226291.jpg

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Adrenal Grafts in the Central Nervous System: Chromaffin and Chromaffin Progenitor Cell Transplantatanimal models and preclinical studies as a source for cell replacement therapy in Parkinson’s disease. The long-term cell survival of these cells in the nervous system is limited, and the observed motor improvements are highly variable. An alternative source for transplantation is chromaffin progeni

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Immunogold for Protein Location in Chromaffin Cellsdate their contribution to cellular function within the adrenal gland and can be achieved only by immunoelectron microscopy. The most popular immunoelectron microscopic techniques involved gold particles conjugated to secondary antibodies, leading to electron-dense markers and the so-called immunogo

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Visualization of Exo- and Endocytosis Membrane Dynamics with Super-Resolution STED Microscopych as fusion pore opening, pore expansion, constriction, and closure, as well as the membrane transformation from flat-shaped to round-shaped vesicles in real time. Here we depict a method of using the state-of-the-art STED microscopy to image these membrane dynamics in bovine chromaffin cells. This

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Real Time Recording of Perifused Chromaffin Cellsin their molecule chemical groups with the ability to yield (oxidize) or capture (reduce) electrons upon its exposure to an electrical field. In order to measure the secretion of catecholamines, they are oxidized at +650?mV with a carbon electrode, releasing every molecule of catecholamine that is o