Titlebook: Chemical and Synthetic Approaches in Membrane Biology; Arun K. Shukla Book 2017 Springer Science+Business Media LLC 2017 Membrane protein

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Chemical and Synthetic Approaches in Membrane Biology

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,Approaches for Preparation and Biophysical Characterization of Transmembrane β-Barrels,tly available protocols for efficient refolding of β-barrel membrane proteins in both lipidic and detergent systems. These protocols are designed to enable the protein to attain its native structural and functional characteristics in any chosen membrane mimetic environment. Further, we detail the cu

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Probing the Dynamics of Plasma Membrane Glutamate Transporters in Real Time by Total Internal Fluor Here we provide a method for using TIRF microscopy to study the basal and regulated trafficking of the excitatory amino acid transporter 3 (EAAT3/EAAC1), a glutamate transporter of the solute carrier family 1 (SLC1). A detailed protocol of how carrying out image recording under TIRF microscopy and

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Single-Molecule Localization Super-Resolution Microscopy of Synaptic Proteins,apted to the culture of neurons from other brain regions. Procedures for handling fixed and live specimens are described, as well as the use of extrinsic fluorescent probes and fluorescent proteins, mounting media, examples of hardware configurations, software for image analysis, and some hints for

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Quantum Dot Toolbox in Membrane Neurotransmitter Transporter Research, principles of subdiffraction-limit fluorescence microscopy and single molecule analysis of transporter surface dynamics. Included in this chapter are three protocols with experimental considerations specific to each technical section.

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Lipid Bilayer Patterns Fabrication by One-Photon Lithography,molecular composition and mobility of this system at micro- and nanoscales have inspired several new routes of biological and biotechnological investigation. Successes in this field include the development of photolithographic and soft lithography techniques which allow the printing of a variety of

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,Approaches for Preparation and Biophysical Characterization of Transmembrane β-Barrels,on transporters to enzymes and signaling molecules. These facets render membrane proteins as ideal drug targets. Despite their significance in biological systems, our knowledge of the structural and functional behavior of membrane proteins remains considerably limited, when compared with their solub