Titlebook: Chemical and Synthetic Approaches in Membrane Biology; Arun K. Shukla Book 2017 Springer Science+Business Media LLC 2017 Membrane protein

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https://doi.org/10.1007/978-1-4939-6836-7Membrane protein structure; Membrane protein dynamics; Drug targets; Novel therapeutics; Lipid membranes

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978-1-4939-8313-1Springer Science+Business Media LLC 2017

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Protein Analysis and Purification Here we provide a method for using TIRF microscopy to study the basal and regulated trafficking of the excitatory amino acid transporter 3 (EAAT3/EAAC1), a glutamate transporter of the solute carrier family 1 (SLC1). A detailed protocol of how carrying out image recording under TIRF microscopy and

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https://doi.org/10.1007/b138330 principles of subdiffraction-limit fluorescence microscopy and single molecule analysis of transporter surface dynamics. Included in this chapter are three protocols with experimental considerations specific to each technical section.

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