Titlebook: Cell-Free Translation Systems; Alexander S. Spirin Book 2002 Springer-Verlag Berlin Heidelberg 2002 Expression.Polymerasekettenreaktion.Pr

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Expression of an Aggregation-Prone Protein in the RTS 500 Systeme and aggregated forms or inclusion bodies. The conventional molecular mechanism of this process suggests that nascent polypeptide chains have two alternative and competitive pathways between folding and aggregation (King et al. 1996). Although solubilization/refolding procedures exist to obtain pro

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Planning and Management Concepts,For a long time, cell-free protein synthesis was more or less an analytical method due to low synthesis rate and productivity. However, several key improvements over the last decades have lead to protocols providing real preparative amounts of protein:

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Miriam Adebibe,Simon W. Coppackd can continue after cell disruption. Thus, disrupted cells or their isolated fractions were reported to be capable of synthesizing proteins (Borsook 1950; Winnick 1950a, 1950b; Siekevitz and Zamecnik 1951; Siekevitz 1952; Peterson and Greenberg 1952; Khesin 1953; Gale and Folkes 1954). In the meant