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Titlebook: Cell-Free Translation Systems; Alexander S. Spirin Book 2002 Springer-Verlag Berlin Heidelberg 2002 Expression.Polymerasekettenreaktion.Pr

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書目名稱Cell-Free Translation Systems
編輯Alexander S. Spirin
視頻videohttp://file.papertrans.cn/223/222948/222948.mp4
圖書封面Titlebook: Cell-Free Translation Systems;  Alexander S. Spirin Book 2002 Springer-Verlag Berlin Heidelberg 2002 Expression.Polymerasekettenreaktion.Pr
描述This is a unique book that describes the most recent achievements in the methodology of protein biosynthesis under cell-free conditions. Various versions of cell-free protein-synthesizing systems and their applications to production of individual proteins on a preparative scale are reviewed. The most recent, advanced methodologies, such as continuous-exchange and continuous-flow cell-free systems and novel effecting batch-format cell-free procedures, are considered. Special attention is drawn to the possibilities of structural (NMR; X-ray) analysis of various gene expression products with the use of a new generation of cell-free systems.
出版日期Book 2002
關(guān)鍵詞Expression; Polymerasekettenreaktion; Protein Expression; Proteinsynthese; RNA; bacteria; biosynthesis; gen
版次1
doihttps://doi.org/10.1007/978-3-642-59379-6
isbn_softcover978-3-642-63956-2
isbn_ebook978-3-642-59379-6
copyrightSpringer-Verlag Berlin Heidelberg 2002
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Yeast Cell-Free Translation Systemsare easy to handle and amenable to very powerful genetic and biochemical analysis, the yeast system represents an attractive model system for studies on the mechanism and regulation of eukaryotic translation. The first cell-free systems capable of initiating translation on exogenous mRNA were prepar
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Poly(A)-Dependent Cell-Free Translation Systems from Animal Cellscial class of RNA molecule: messenger RNA. Extracts derived from rabbit reticulocytes, Krebs II ascites cells and HeLa cells were instrumental in the identification of messenger RNA as the ultimate carrier of information to the protein synthesis machinery (Labrie 1969). Subsequently, in vitro system
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Continuous-Flow and Continuous-Exchange Cell-Free Translation Systems and Reactors expression of foreign genes in bacterial or eukaryotic cells are efficient and widely used. However, difficulties associated with cytotoxicity, proteolytic degradation or improper folding and aggregation of synthesized proteins are often encountered .. The . expression systems are restricted by mec
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