Titlebook: Cell Reprogramming for Immunotherapy; Methods and Protocol Samuel G. Katz,Peter M. Rabinovich Book 2020 Springer Science+Business Media, LL

Arbitrary

Microfluidic Approach for Highly Efficient Viral Transductionfluidic devices for efficient ex vivo gene transfer. Up to four- to fivefold reductions in viral vector usage and two- to fourfold reductions in transduction times can be obtained by using this method.

CLOWN

Single-Cell Cytokine Analysis to Characterize CAR-T Cell Activationo study their heterogeneous cytokine response at the single-cell level following activation. Here, we describe an activation platform which incubates single CAR-T cells with single-target CD19 antigen-presenting cells (APCs) and measures the resulting cytokine secretions. This can be compared to the

大約冬季

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Minikin

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cellsnthetic” genes. NK cells represent 10–20% of total lymphocytes in the peripheral blood of humans and play an essential role in clearing infections and malignant cells. A significant number of NK cells express and utilize non-clonotypic receptors that recognize cognate ligands expressed on a broad sp

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OCTO

Immunomodulation of NK Cell Activity modulate the activity of different cells of the immune system. The recognition of target cells is mediated by germ line-encoded receptors, and the activity of NK cells can be further regulated by soluble factors such as cytokines and Toll-like receptor ligands. Thus, NK cells display an exciting po

紳士

An Overview of Advances in Cell-Based Cancer Immunotherapies Based on the Multiple Immune-Cancer Celsystemic levels. A number of studies indicate that various immune cells participate in tumor development (Fig. .). In this review, we will discuss interactions among T lymphocytes (T cells), B cells, natural killer (NK)?cells, dendritic cells (DCs), tumor-associated macrophages (TAMs), neutrophils,

EWE

Rapid Production of Physiologic Dendritic Cells (phDC) for Immunotherapyarrow precursors or human?blood monocytes to DC via cultivation with supraphysiologic concentrations of cytokines such as GM-CSF and IL-4 for up to 7?days. Alternatively, our group has recently established a new approach, based on the underlying mechanism of action of a widely used cancer immunother