Titlebook: Cell Reprogramming for Immunotherapy; Methods and Protocol Samuel G. Katz,Peter M. Rabinovich Book 2020 Springer Science+Business Media, LL

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The Propagation and Quantification of Two Emerging Oncolytic Viruses: Vesicular Stomatitis (VSV) andses vesicular stomatitis virus and Zika virus. Here, we describe methods to propagate and titer these two viruses. We also describe a simple cell-killing assay to begin testing modified viruses for increased potential killing of glioblastoma cells.

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Engineering of Natural Killer Cells for Clinical Applicationin technologies that allow the production of large numbers of highly potent cells with specific anticancer activity. Here, we describe clinically applicable protocols for NK cell engineering, including expansion of NK cells and genetic modification using electroporation of messenger RNA.

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Book 2020aboratory protocols, and tips on troubleshooting and avoiding known pitfalls...?Authoritative and cutting-edge, .Cell Reprogramming for Immunotherapy: Methods and Protocols .aims to ensure successful results in the further study of this vital field..

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I F Publications on ferroelectrics,irecting and activating T cells toward attacking EGFR-expressing cancer cells, the designed SMART-Exos exhibit highly potent and specific antitumor activity. In this chapter, the methodologies are outlined for generating and using SMART-Exos for cancer immunotherapy.

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Testing the Specificity of Compounds Designed to Inhibit CPT1A in T Cellsimmunotherapy. Here, we combine the analysis of posttranscriptional gene silencing with extracellular flux assays to determine if etomoxir exerts nonspecific effects on oxidative metabolism. The off-target effects of ETO that we describe highlight the challenges of using pharmacologic inhibitors in loss-of-function approaches in T cells.