Titlebook: Caspases,Paracaspases, and Metacaspases; Methods and Protocol Peter V. Bozhkov,Guy Salvesen Book 2014 Springer Science+Business Media New Y

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Corporeal

Cardiac-Output

Invertebrate

Preparation of , Seedling Proteomes for Identifying Metacaspase Substrates by N-terminal COFRADIC by comparing the N-terminal proteomes (or N-terminomes) of wild-type plants to transgenic plants not expressing a given metacaspase. In this chapter we describe a protocol for the preparation of plant tissue proteomes, including differential isotopic labelling allowing for a comparison of in vivo N

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ASSET

PANIC

Peter V. Bozhkov,Guy SalvesenIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the expert.Includes supplementary material

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Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/222387.jpg

GUILT

Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Spscribe the protocol for analyzing S4-S2 pockets preferences of caspases using PS-SCL. Additionally, we describe procedures for the identification of optimal substrates sequence after PS-SCL, solid phase synthesis, and purification of selected fluorogenic substrates, as well as their kinetic analysis.

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