Titlebook: Caspases,Paracaspases, and Metacaspases; Methods and Protocol Peter V. Bozhkov,Guy Salvesen Book 2014 Springer Science+Business Media New Y

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Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Spscribe the protocol for analyzing S4-S2 pockets preferences of caspases using PS-SCL. Additionally, we describe procedures for the identification of optimal substrates sequence after PS-SCL, solid phase synthesis, and purification of selected fluorogenic substrates, as well as their kinetic analysis

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Methods for the Study of Caspase Activation in the , Oocyte and Egg Extractificantly contributed to these advances. Twenty years ago, Newmeyer and colleagues first showed that the . egg extract, when incubated at room temperature, reconstituted the key molecular events of cellular apoptosis including cytochrome . release, nuclear condensation, internucleosomal fragmentatio

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Caspase Protocols in Mice apoptotic pathway. Since the discovery of caspases, many methods have been developed to detect their activation and are widely used in basic and clinical studies. In a mouse tissue, caspase activation can be monitored by cleavage of caspase-specific synthetic substrates and by detecting cleaved cas

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Measurement of Caspase Activation in Mammalian Cell Culturesleaving protein substrates harboring specific target motifs. Basically all biochemical and morphological changes in an apoptotic cell, including cell shrinkage, chromatin condensation, DNA fragmentation, and plasma membrane blebbing, are consequence of caspase-mediated proteolysis. Thus, uncovering

CLASP

Detection and Measurement of Paracaspase MALT1 Activity the immune response. Oncogenic activation of MALT1 is associated with the development of specific forms of B-cell lymphomas. Through specific cleavage of its substrates, MALT1 controls various aspects of lymphocyte activation, including the activation of transcriptional pathways, the stabilization

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Metacaspase: An Arginine-Specific Peptidaseole of metacaspase in . is still a matter of debate, whereas its peptidase enzymatic activity has been well characterized. Among the different possible expression systems, metacaspase-deficient yeast cells (.) have been instrumental in studying the activity of . metacaspase (LmjMCA). Here, we descri