Titlebook: Cardiac Regeneration; Methods and Protocol Kenneth D. Poss,Bernhard Kühn Book 2021 Springer Science+Business Media, LLC, part of Springer N

流動性

Aidan Norrie,Carolyn Harris,Elena Woodacreation are monitored in real time after uninjured or injured hearts are excised, washed, and cultured for up to 30?days. In addition to these techniques, we describe ex vivo genetic ablation of the epicardium, cell proliferation assays, partial ventricular explant culturing, and chemical screening.

不如樂死去

Differentiation of Human Induced Pluripotent Stem Cells into Epicardial-Like Cellstic of the developing epicardium. We also review our protocol for differentiating iECs into fibroblasts and smooth muscle cells through treatment with bFGF and TGF-β1, respectively. iECs provide a platform for studying fundamental epicardial biology and can inform strategies for therapeutic heart regeneration.

引水渠

在駕駛

障礙物

https://doi.org/10.1007/978-3-319-74048-5njury models and describe a set of methods used to accurately analyze and quantify cardiac outcomes. Importantly, a comprehensive understanding of cardiac regeneration and repair requires a combination of multiple functional, histological, and molecular analyses.

fastness

Ringworm

https://doi.org/10.1057/9780230109056ventional methods, while the hCMP fabrication procedure produces a patch of clinically relevant size and incorporates a simple method for maturing the engineered tissue via mechanical stimulation. We also describe how the patch can be evaluated in a large-animal (swine) model of myocardial injury.

Vasodilation

Normative Aspekte der Public Relationstes. Moreover, we describe a cardiac differentiation protocol to derive cardiomyocytes from hiPSCs, as well as a strategy to use fluorescence-activated cell sorting to isolate pure populations of fluorescently labeled cardiomyocytes for downstream applications.

FER

Indigence