Titlebook: Cardiac Regeneration; Methods and Protocol Kenneth D. Poss,Bernhard Kühn Book 2021 Springer Science+Business Media, LLC, part of Springer N

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Differentiation of Human Induced Pluripotent Stem Cells into Epicardial-Like Cells and smooth muscle populations of the heart and secrete paracrine factors that promote cardiomyocyte proliferation and angiogenesis. Despite a central role in cardiac biology, the mechanisms by which epicardial cells influence cardiac growth are largely unknown, and robust models of the epicardium a

羽毛長成

GULF

Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs. Here, we describe a versatile “frame-hydrogel” methodology to generate engineered cardiac tissues with highly mature functional properties. This methodology has been successfully utilized with a variety of cell sources (neonatal rat ventricular myocytes, human and mouse pluripotent stem cell-deriv

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Efficient Protocols for Fabricating a Large Human Cardiac Muscle Patch from Human Induced Pluripotendisease; however, their full potential cannot be met without robust methods for differentiating them into cardiac-lineage cells. Here, we present novel protocols for generating hiPSC-derived cardiomyocytes (CMs), endothelial cells (ECs), and smooth muscle cells (SMCs) and for assembling them into a

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曲解

Ex Vivo Techniques to Study Heart Regeneration in Zebrafishanisms of cardiac regeneration. Upon injury, the epicardium, the outermost mesothelial tissue layer of vertebrate hearts, serves dual purposes in the regenerating heart as both a signaling center and a source for crucial cell types. Traditional in vivo genetic approaches to study heart regeneration

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https://doi.org/10.1057/9780230109056actors, with tissues routinely reaching conduction velocities and specific forces of 25?cm/s and 20?mN/mm., respectively, and forces per input cardiomyocyte of up to 12?nN. This method is reproducible and readily scalable to generate small tissues ideal for in vitro testing as well as tissues with large, clinically relevant dimensions.