Titlebook: Carbohydrate-Based Vaccines; Methods and Protocol Bernd Lepenies Book 2015 Springer Science+Business Media New York 2015 AutoMap software.C

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Temporary Conversion of Protein Amino Groups to Azides: A Synthetic Strategy for Glycoconjugate Vacreaction with carboxylic acid side chains is used to attach amino tether groups of a ligand to the protein. Azide-alkyne Huisgen cycloaddition conjugation, “click chemistry” is used to attach a second ligand equipped with a propargyl group or an analogous terminal alkyne, and following reduction of

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Characterization of Carbohydrate Vaccines by NMR Spectroscopy,ce (NMR) spectroscopy has been established as an extremely useful and robust method for tracking the industrial manufacturing process of these vaccines from polysaccharide bulk antigen through to the final formulation. Here, we describe the use of proton NMR for structural identity and conformity testing of carbohydrate-based vaccines.

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Carbohydrate-Based Vaccines978-1-4939-2874-3Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Non-Aligned Psychiatry in the Cold Warce (NMR) spectroscopy has been established as an extremely useful and robust method for tracking the industrial manufacturing process of these vaccines from polysaccharide bulk antigen through to the final formulation. Here, we describe the use of proton NMR for structural identity and conformity testing of carbohydrate-based vaccines.

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https://doi.org/10.1007/978-1-4939-2874-3AutoMap software; Carbohydrates; carbohydrate antigens; glycan microarray fabrication; glycans; glycoconj

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