Titlebook: Carbohydrate-Based Vaccines; Methods and Protocol Bernd Lepenies Book 2015 Springer Science+Business Media New York 2015 AutoMap software.C

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The Glycan Array Platform as a Tool to Identify Carbohydrate Antigens,es by mass spectrometry is difficult, and detection reagents, such as monoclonal antibodies and lectins, are only available for a small subset of glycans. An alternative approach involves profiling serum anti-glycan antibody populations to identify unique antibodies or changes in antibody subpopulat

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CARK

Generation of Monoclonal Antibodies against Defined Oligosaccharide Antigens, is a frequent feature of cancer cells. Antibodies recognizing such carbohydrate antigens may be used for the specific detection of potentially harmful cells, immunohistochemistry, and diagnostic and therapeutic applications. The generation of specific and strongly binding antibodies against defined

過(guò)時(shí)

Murine Whole-Blood Opsonophagocytosis Assay to Evaluate Protection by Antibodies Raised Against Enc) are preferred to ELISA titers for evaluating protection against infection. Such assays are normally performed using phagocytic cell lines or purified cell types, which underestimate the complexity of blood bactericidal activity. Here, we describe an OPA using murine whole-blood as effector cells,

過(guò)時(shí)

Determination of ,-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry anscribe how ion mobility mass spectrometry can be used to obtain spectra from virion samples. We also describe how negative ion fragmentation of glycans can be used to probe structural features of virion glycans.

Lasting

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Temporary Conversion of Protein Amino Groups to Azides: A Synthetic Strategy for Glycoconjugate Vacto screen lectins, toxins, and antibodies. A novel approach to potentiate and profile the immune response to vaccines involves targeting antigens directly to dendritic cells (DCs), the key cells engaged in the immunization process. Inclusion of a carbohydrate ligand recognized by C-type lectins expr

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