Titlebook: Cancer Genomics and Proteomics; Methods and Protocol Paul B. Fisher Book 2007 Humana Press 2007 BAC.Carcinom.Chromosom.DNA.Microarray.Trans

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書目名稱Cancer Genomics and Proteomics影響因子(影響力)




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Cloning Differentially Expressed Genes Using Rapid Subtraction Hybridization (RaSH), differentially expressed genes represent critical elements in this process. Many techniques have been developed to facilitate achieving these objectives. Although effective in many situations, most currently described approaches are not trouble-free and have limitations, including complexity of per

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The Application of Differential Display as a Gene Profiling Tool,fications that have been described over the last several years. A highly reproducible, semihigh-throughput differential display protocol used in our laboratories is described along with an example of its successful application using pancreatic cancer cells. In addition to the work performed in our l

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Gene Expression Profile Analysis of Tumors,les for all known genes in the human genome. The genome-wide analysis of the gene expression patterns of neoplastic and normal cells provides insights into: (1) the identification of previously unknown tumor subtypes; (2) the normal cellular counterparts of tumor cells; (3) the identification of cel

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Complete Open Reading Frame (C-ORF) Technique,CR. However, these protocols are not infallible and in specific instances they have proven unsuccessful, emphasizing a need for further refinement. A novel method, the complete open reading frame (C-ORF) technique, is presently described, which has proven successful in cases, where standard rapid am

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Chromatin Immunoprecipitation Assays,es, their downstream targets, or owing to increased proliferation, and altered apoptotic potential. Various microarray based techniques have been widely utilized to study the differential expression of genes in cancer in recent years. Along with this, attempts have been made to study the transcripti

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Manipulating Genes and Gene Copy Number by Bacterial Artificial Chromosome Transfection,nd other large genomic loci. Protocols and parameters that influence the efficient transfection of these large DNA molecules into cells in culture were described here. By carefully optimizing the conditions for the formation of compact transfection complexes, BACs can be introduced into a variety of