Titlebook: Cancer Cell Biology; Methods and Protocol Sherri L. Christian Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive li

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Evaluating Cell Membrane Localization and Intracellular Transport of Proteins by Biotinylation,Abnormal protein localization can affect key cell functions, including transduction of extracellular signals and organization of the cytoskeleton, significantly affecting oncogenicity and therapeutic responses. In this chapter, we describe a surface protein biotinylation method that allows the study

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The Use of Seahorse XF Assays to Interrogate Real-Time Energy Metabolism in Cancer Cell Lines,uring cellular metabolism is therefore central to understanding the cellular physiology of cancer cells. The Seahorse XFe Analyzer series allows real-time measurement of cellular metabolism. In the basic assay, two parameters, the oxygen consumption rate (OCR) and the extracellular acidification rat

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1064-3745 ology: Methods and Protocols.?aims?to?provide a?..comprehensive set of tools for the analysis of cancer cell biology in the lab..978-1-0716-2378-7978-1-0716-2376-3Series ISSN 1064-3745 Series E-ISSN 1940-6029

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,Flow Cytometry Analysis to Detect Lapatinib-Induced Modulation of Constitutive and IFN-γ-Induced HLy data clearly show lapatinib-mediated downregulation of both constitutive and IFN-γ-induced HLA class I expression. These results, for which we do not yet have an explanation, may have important implications for our understanding of lapatinib resistance in metastatic HER2+ cancer.

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Manual Immunofluorescence of Formalin-Fixed Paraffin-Embedded Human Tumor Tissues,y and highly accessible method for immunofluorescence of FFPE-embedded tissues, hoping to enable more laboratories to take on this highly informative technique and empower them to begin IF analysis in their own tissues of interest.

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The Use of Seahorse XF Assays to Interrogate Real-Time Energy Metabolism in Cancer Cell Lines,e Seahorse Real-Time ATP Rate Assay for adherent and suspension cancer cell lines. Depending on individual experimental results, more refined subsequent assays can then be performed to specifically determine, for example, the ability to utilize different substrates by the cell lines in the presence