Titlebook: Cancer Cell Biology; Methods and Protocol Sherri L. Christian Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive li

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Processing and Cryopreservation of Blood, Cancer Tissues, and Cancer Cells for Viable Biobanking,ions of these materials, however, do not enable the establishment of short-term cultures, cell lines, or patient-derived xenograft models for functional studies. Also, intact dissociated cells that are required for some single-cell analyses cannot be obtained from these material types. Adding viable

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,Adipocyte–Breast Cancer Cell Co-Culture in Transwells,proximity. Here we describe co-culture of Simpson–Golabi–Behmel syndrome (SGBS) adipocyte cells with the MCF-7 breast cancer cell line using the Corning. Transwell. 12-mm, 0.4-μm pore polyester membrane insert cell culture system. The SGBS adipocyte cell line, which was developed from cells taken fr

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Enzyme-Linked Immunosorbent Assay (ELISA),ith advances in analytical methods, ELISA assay has been constantly optimized to improve its sensitivity, and different types of ELISA are now available to detect various biomarkers. This chapter provides an overall summary of the basic principle of ELISA, discusses different components of ELISA ass

intricacy

,Flow Cytometry Analysis to Detect Lapatinib-Induced Modulation of Constitutive and IFN-γ-Induced HL number of positive cells and their expression levels. Loss or downregulation of HLA-I molecules on cancer cells, a well-documented immune escape mechanism, may occur via activation and integration of numerous signalling pathways that are operative in cancer. Whereas IFN-γ, produced during an adapti

Influx

An End-to-End Workflow for Interrogating Tumor-Infiltrating Myeloid Cells Using Mass Cytometry,ts can impact tumor progression differently, with conventional type 1 DCs important in protective anti-tumor immune responses, while immunosuppressive tumor-associated macrophages and myeloid-derived suppressive cells (MDSCs) play tumor-promoting roles. Deep phenotyping of myeloid cells using single

inclusive

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