Titlebook: Calcium-Binding Proteins of the EF-Hand Superfamily; From Basics to Medic Claus W. Heizmann Book 2019 Springer Science+Business Media, LLC,

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Fecal-Impaction

The Use of Cre/loxP Inducible Mouse Models to Dissect the Specific Roles of Calcineurin Signaling ints. Calcineurin is activated through increased intracellular levels of calcium that is triggered by cell receptors. The phosphatase activity of calcineurin dephosporylates the members of NFAT transcription factor family and activates their translocation to the nucleus and further starts the transcri

Fester

Calpain Purification Through Calpastatin and Calcium: Strategy and Procedures reversible binding properties of calpain’s intrinsically disordered protein (IDP) inhibitor, calpastatin. IDPs are easily produced in heterologous expression hosts and purified to homogeneity. Combining these properties with in vivo biotinylation leads to a simplified purification strategy whereby

Presbycusis

Characterization of the EF-Hand Calcium-Binding Domains of Human Plastinsg proteins. Plastins are involved in the regulation of the actin cytoskeleton as well as the cross-linking of actin filaments. In addition to four calponin-homology (CH) domains, all three plastins contain two N-terminal EF-hand Ca.-binding motifs which together are homologous to a single lobe of th

Obloquy

Expression and Characterization of MICU2, a Ca2+ Sensor Proteineath, metabolism, and cytoplasmic Ca. signaling. Here we describe the procedures for protein preparation of various MICU2 constructs, which have enabled successful in vitro characterizations of MICU2 including interaction with MICU1 using pull-down assays and oligomerization using multi-angle laser

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Patientenbezogene Dokumentationvalbumins with strategically designed EF-hand motifs in adult cardiac myocytes via acute adenoviral gene transfer. These protocols have been used extensively to optimize parvalbumin function as a potential therapeutic for failing heart muscle.

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Frank Studenski,Wolfgang Münsternt role of calcineurin/NFAT pathway in orchestrating the developmental and immune response processes. This protocol describes several examples of the use of Cre/loxP inducible mouse models deficient in calcineurin in different myeloid subsets to dissect the cell- and tissue-specific roles of calcineurin signaling.

CON

Wilhelm Knelangen,Johannes Varwick ITC studies, and NMR interaction studies with different peptides and calcium. In combination, these three?experimental techniques provide detailed insights into a novel regulatory mechanism, involving the linker region between the EF-hand domain and the first CH domain?of the plastins.

sphincter

Gene Transfer of Calcium-Binding Proteins into Adult Cardiac Myocytesvalbumins with strategically designed EF-hand motifs in adult cardiac myocytes via acute adenoviral gene transfer. These protocols have been used extensively to optimize parvalbumin function as a potential therapeutic for failing heart muscle.

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