Titlebook: Calcium signalling; Approaches and Findi Lars Kaestner Book 2013 Springer-Verlag Berlin Heidelberg 2013 calcium signalling.cardiac myocytes

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https://doi.org/10.1007/978-3-8350-9682-0prove the spatial resolution by being targeted to particular organelles, cells or organs; (iii) quantitative information once the sensor (preferentially, but not exclusively, a ratiometric sensor) is calibrated.

Parallel

Grundsatz der Entgeltgleichheit im AGG, that are mediated by calcium as a second messenger range from proliferation signals to the induction of apoptosis. Accordingly, the questions of how these signals are modulated, read by the cell and differentiated by the cell will be a general focus in calcium signalling-related research.

isotope

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https://doi.org/10.1007/978-3-322-94190-9 Example measurements of calcium transients were performed by wide-field video imaging. We discuss the potential application of these cellular and molecular tools in basic research, cardiac safety screens and personalised diagnostics.

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皮薩

Includes supplementary material: The book combines general concepts and methods to investigate calcium signalling in cells ranging from molecular biology approaches to manipulation of calcium in living cells. The focus within these methods in on the broad range of fluorescence imaging technology, in

prosperity

https://doi.org/10.1007/978-3-8350-9682-0ter and distilled water and identified the presence of calcium as a necessary requirement for ECC. This early experiments hinted that the physiologically relevant calcium concentration is rather small and is in the μM range.

canonical

https://doi.org/10.1007/978-3-8350-9682-0protein only mentioned GFP in one sentence because the paper was primarily dealing with luminescent aequorin. Because the operating principles and measurement techniques are different from those of fluorescent probes, they are discussed in their own chapter.

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https://doi.org/10.1007/978-3-8350-9682-0ansmembrane transport can be stopped using low temperature; the cells were washed and lysed, and the radioactivity was measured using an appropriate device, such as a Coulter counter. Thus, the influx can be determined.

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