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Titlebook: Calcium signalling; Approaches and Findi Lars Kaestner Book 2013 Springer-Verlag Berlin Heidelberg 2013 calcium signalling.cardiac myocytes

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發(fā)表于 2025-3-21 16:17:37 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Calcium signalling
副標題Approaches and Findi
編輯Lars Kaestner
視頻videohttp://file.papertrans.cn/221/220811/220811.mp4
概述Broad topical coverage from general concepts to detailed examples.96 Figures and 4 Tables.Includes latest developments and research results.Includes supplementary material:
圖書封面Titlebook: Calcium signalling; Approaches and Findi Lars Kaestner Book 2013 Springer-Verlag Berlin Heidelberg 2013 calcium signalling.cardiac myocytes
描述The book combines general concepts and methods to investigate calcium signalling in cells ranging from molecular biology approaches to manipulation of calcium in living cells. The focus within these methods in on the broad range of fluorescence imaging technology, in particular on optical sectioning techniques and fast image acquisition. In addition to these general guidelines there are application examples in a context beyond calcium signalling in two major fields: investigations of isolated cardiac myocytes and red blood cell related research. While the cellular cardiology section provides snapshots of certain calcium signalling aspects, the red blood cell part presents an overview from the functional identification of calcium-channels to a concept of physiological and pathophysiological relevance.
出版日期Book 2013
關(guān)鍵詞calcium signalling; cardiac myocytes; erythrocytes; optical imaging; red blood cells; Biological Microsco
版次1
doihttps://doi.org/10.1007/978-3-642-34617-0
isbn_softcover978-3-642-34616-3
isbn_ebook978-3-642-34617-0
copyrightSpringer-Verlag Berlin Heidelberg 2013
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https://doi.org/10.1007/978-3-8350-9682-0covered the essential role of calcium in the process that is now named excitation–contraction coupling (ECC). Ringer performed experiments with tap water and distilled water and identified the presence of calcium as a necessary requirement for ECC. This early experiments hinted that the physiologica
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https://doi.org/10.1007/978-3-8350-9682-0ted with relatively minimal disturbance. Fluorescence-based methods can deliver the following information: (i) a high temporal resolution, down to the order of milliseconds; (ii) spatial resolution down to diffraction-limited spots, and in the future, potentially even lower. The sensor itself can im
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https://doi.org/10.1007/978-3-8350-9682-0plex (GFP, green fluorescent protein) that naturally occurs in the jellyfish .. Interestingly, the famous paper by Shimomura that identified GFP as a protein only mentioned GFP in one sentence because the paper was primarily dealing with luminescent aequorin. Because the operating principles and mea
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https://doi.org/10.1007/978-3-8350-9682-0d above), there are many techniques that can be used to identify an unknown player. This biochemical and molecular biology-based methodology is reviewed in other sources. Examples include high-pressure liquid chromatography (HPLC), 2D-gel electrophoresis, mass spectrometry, matrix-assisted laser des
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