Titlebook: CRISPR Guide RNA Design; Methods and Protocol Tudor A. Fulga,David J. H. F. Knapp,Quentin R. V. Book 2021 Springer Science+Business Media,

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Controlling the Activity of CRISPR Transcriptional Regulators with Inducible sgRNAsthe presence of a user defined inducer. In this chapter, I cover the know-how relating to the design and synthesis of iSBH-sgRNAs, as well as the implementation in mammalian cells of inducible CRISPR-TR strategies based on this technology.

萬靈丹

Gene Manipulation Using Fusion Guide RNAs for Cas9 and Cas12as of the CRISPR-Cas system. Recently, we have developed fusion guide RNAs (fgRNAs) for orthogonal gene manipulation using Cas9 and Cas12a. Here, we describe the methods for designing and generating fgRNAs-expression constructs to achieve multiplex genome editing and gene manipulation in human cells.

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獨白

The Thin Filament in Insect Flight Muscleprovide a detailed protocol for use of CRISPR-Cpf1 and chemically modified guide RNAs in cell lines, outlining the steps from designing guide RNAs to a target gene of interest, delivery and expression in cells, and analysis of gene editing events.

cravat

The Thin Filament in Insect Flight Muscle active sgRNA. The benefits of our approach lie in the stringent purification of the DNA-targeting 20-mer, the reduced synthesis of the constant 79-mer each time a new sgRNA is required, and the rapid access it provides to custom libraries of sgRNAs.

heterogeneous

Noch einmal: Geschichte in Schichtgesteinen,identify off-target cleavage events throughout an entire genome. Together, these techniques can be used to assess the reliability of experimental models generated using CRISPR/Cas9 as well as the safety of therapeutics employing this technology.

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轎車

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