Titlebook: CRISPR Guide RNA Design; Methods and Protocol Tudor A. Fulga,David J. H. F. Knapp,Quentin R. V. Book 2021 Springer Science+Business Media,

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書目名稱CRISPR Guide RNA Design影響因子(影響力)




書目名稱CRISPR Guide RNA Design影響因子(影響力)學科排名




書目名稱CRISPR Guide RNA Design網(wǎng)絡公開度




書目名稱CRISPR Guide RNA Design網(wǎng)絡公開度學科排名




書目名稱CRISPR Guide RNA Design被引頻次




書目名稱CRISPR Guide RNA Design被引頻次學科排名




書目名稱CRISPR Guide RNA Design年度引用




書目名稱CRISPR Guide RNA Design年度引用學科排名




書目名稱CRISPR Guide RNA Design讀者反饋




書目名稱CRISPR Guide RNA Design讀者反饋學科排名

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978-1-0716-0689-6Springer Science+Business Media, LLC, part of Springer Nature 2021

Jingoism

Tudor A. Fulga,David J. H. F. Knapp,Quentin R. V. Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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https://doi.org/10.1057/9780230245099onsiderations for the design of short guide (sg) RNAs include the assessment of possible off-target activities, the prediction of on-target efficacies and mutational outcome. Manual design of sgRNAs taking into account these parameters, however, remains a difficult task. Thus, computational tools to

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https://doi.org/10.1057/9780230245099ine, and gene therapy. However, CRISPR nucleases can cleave off-target sites as well as on-target sites, causing unwanted mutations. Furthermore, after CRISPR treatments are delivered into cells or organisms, it is important to estimate the resulting mutation rates and to determine the patterns of m

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The Thin Filament in Insect Flight Muscleing promise as a therapeutic for patients with genetic diseases. Multiple different CRISPR systems have been identified, each with its own target DNA recognition sequence, expanding the editable mammalian genome. It is also now appreciated that chemically modified nucleic acids can substitute for un

脫毛

The Thin Filament in Insect Flight Muscleby enzymatic reactions, however the process becomes time-consuming as the number of sgRNAs increases and does not allow the incorporation of chemical modifications that can improve or expand the functionality of CRISPR. Solid-phase RNA synthesis can overcome these issues, but highly pure full-length